Culture Age, Growth Medium, Ultrasound Amplitude, and Time of Exposure Influence the Kinetic Growth of Lactobacillus acidophilus

Abstract

The growth pattern of probiotics can be modified by changing their nutritional factors and their physiological stage. Meanwhile, high intensity ultrasound (HIUS) can be employed to increase probiotics’ biomass. The one-factor-at-a-time (OFAT) approach was employed to investigate the influence of the growth medium (MRS broth, whole milk, and skim milk), culture age (1 day and 7 days old) and ultrasound parameters (time and amplitude) on the kinetic parameters of L. acidophilus. The oldest culture (7 days) had a greater lag phase and time to reach the end of the sigmoidal curve (Tmax) (p < 0.05) as well as a lower rate (maximum growth potential μmax) compared to the youngest culture (1 day). Regarding the growth medium, skim milk presented the greatest L. acidophilus counts (p < 0.05). Meanwhile, sonication times (60 and 90 s) change µmax and Tmax. When 30% amplitude was applied, a greater μmax and a smaller Tmax were observed (p < 0.05). It can be concluded that the growth medium, culture age, and ultrasound parameters (time and amplitude) influence the kinetic parameters of L. acidophilus. Results from this study could be used in the design and optimization of processes to improve the growth of the probiotic L. acidophilus at industrial scale.