Three‐Dimensional Lipid Distribution of a Brown Rice Kernel

Abstract

ABSTRACT: Lipid distribution was successfully observed in a brown rice kernel ( Oryza sativa L.) 3‐dimension‐ally (3D) by means of a virtual 3D visualizing model. Sections of an untreated rice kernel were collected on an adhesive tape with preservation of its shape. The actual distribution of lipid was visualized by staining. A virtual 3D visualizing model of the lipid distribution was produced from the stained sequential sections of the rice kernel. Lipid is not only located at the outer layer of the rice kernel but also in lower tissues beneath the seed coat and around the embryo. Lipid distribution at dorsal and ventral sides could also be visualized.