Optimization of subcritical water extraction of polysaccharides from Grifola frondosa using response surface methodology

Abstract

THE OPTIMAL EXTRACTION CONDITIONS INCLUDE: extraction temperature of 210°C, extraction time of 43.65 min and the ratio of water to raw material of 26.15:1. Under these optimal conditions, the experimental yield of the polysaccharides (25.1 ± 0.3%) corresponded with the mean value predicted by the model and two times more than the mean value obtained by the traditional HWE. The antioxidant activities of polysaccharides extracted by SWE were generally higher than those extracted by HWE. From the study, the SWE technology could be a time-saving, high yield, and bioactive technique for production of polysaccharides.