Rapid and efficient high-performance liquid chromatography-ultraviolet determination of total amino acids in protein isolates by ultrasound-assisted acid hydrolysis

Abstract

> 0.999), accuracy (recovery between 80-118 %), and precision (RSD<10.9). The analysis of 15 plant-based proteins revealed distinct amino acid profiles. Compared to traditional acid hydrolysis methods, the ultrasound-assisted approach demonstrated no significant difference in results (p-value > 0.05), confirming its reliability. The optimized ultrasound-assisted method is a reliable and efficient alternative for amino acid analysis, offering significant cost and time savings while maintaining high analytical performance. These findings are crucial for nutritional planning and developing functional foods to improve health outcomes.