Polyphenolic Characterization and Antioxidant Capacity of Laurus nobilis L. Leaf Extracts Obtained by Green and Conventional Extraction Techniques

Abstract

Laurus nobilis L. is an evergreen Mediterranean shrub whose leaves have been known for various health-promoting effects mainly attributed to polyphenols. Microwave- (MAE) and ultrasound-assisted extraction (UAE) are green extraction techniques that enable effective isolation of polyphenols from plant material. Therefore, the aim of this research was to optimize the extraction conditions of MAE (ethanol percentage, temperature, extraction time, microwave power) and UAE (ethanol percentage, extraction time, amplitude) of polyphenols from Laurus nobilis L. leaves and to assess their polyphenolic profile by ultra performance liquid chromatography- tandem mass spectrometry (UPLC-MS/MS) and antioxidant capacity by oxygen radical absorbance capacity (ORAC) assay. Optimal MAE conditions were 50% ethanol, 80 °C, 10 min and 400 W. Optimal UAE conditions were 70% ethanol, 10 min and 50% amplitude. Spectrophotometric analysis showed the highest total phenolic content in the extracts was obtained by MAE, compared to conventional heat-reflux extraction (CRE) and UAE. The polyphenolic profile of all obtained extracts included 29 compounds, with kaempferol and quercetin glycosides being the most abundant. UPLC-MS/MS showed the highest total phenolic content in the extracts obtained by CRE. ORAC assay showed the highest antioxidant capacity in extracts obtained by CRE, which is in agreement with the polyphenolic profile determined by UPLC-MS/MS.