Identification of Antioxidants in Young Mango Leaves by LC-ABTS and LC-MS

Abstract

Thai eat the young leaves of mango as vegetables. Antioxidants in young leaves of mango cultivars 'Talapnak', 'Chok Anan' and 'Nam Dok Mai' were identified by high-performance liquid chromatography coupled with an online ABTS assay (HPLC-ABTS) and electrospray ionization mass spectrometer (HPLC-ESI-MS). Young leaves of mango cv. 'Nam Dok Mai' had the highest antioxidant capacity. Major antioxidants in young mango leaves were man- giferin and benzophenones (maclurin and iriflophenone derivatives). Mangiferin presented in higher quantities than other compounds in each cultivar, with cv. 'Talapnak' containing the most (37.92±0.98 mg/g DW). The compound with the highest antioxidant capacity in all cultivars was mangiferin pentoside (from 1.19±0.25 mmol TE/g DW in cv. 'Chok Anan' to 2.13±0.04 mmol TE/g DW in cv. 'Talapnak'). The compound with the highest Trolox equivalent antioxidant capacity was maclurin galloyl glucoside (1.75±0.62 mol TE/mol).