Research on the structural characteristics of a novel <i>Chinese Iron Yam</i> polysaccharide and its gastroprotection mechanism against ethanol-induced gastric mucosal lesion in a BALB/c mouse model

Abstract

) showed gastric mucosa protective effects on the BALB/c mouse model. The lesion index and lesion inhibition rate of the CIYP and cimetidine treatment groups were significantly altered compared with the ethanol-induced gastric mucosal lesion (GML) group. Moreover, the administration of CIYP showed definite effects of increasing the NO, PGE2 and EGF levels, and SOD activities, and reducing the MDA levels of gastric mucosa tissues to prevent gastric oxidative stress. Histopathological analysis indicated that the microscopic morphology of gastric mucosal tissues was changed after being damaged by ethanol and the damage was significantly reduced after CIYP administration. Finally, the western blot and quantitative real-time polymerase chain reaction (qRT-PCR) results provided comprehensive evidence that the CIYP could repress gastric inflammation through the reduction of IL-1β, TNF-α and IL-6, prevent gastric oxidative stress through the inhibition of lipid peroxides, and favor cell survival via downregulating the TAK1, MKK3, P-p38 and Bax levels and upregulating the protein expression levels, compared with the CIM group.