Valorization of winged bean (Psophocarpus tetragonolobus (L) DC) by evaluation of its antioxidant activity through chemometric analysis

Abstract

Winged bean being a minor pulse crop has been less focused for research purposes. However, seeing at its enormous potential as a nutrient-rich crop, a study was conducted at ICAR-IIVR, Varanasi to encourage this underutilized legume crop. Nineteen winged bean genotypes were evaluated for their antioxidant potentiality and phenol and flavonoids content. Two chemometric techniques (PCA and AHC) were implied to find out the pattern of variation available for antioxidant potentiality as well as to find out the best performing genotypes. The phenol content ranged from 48.4 to 143.5 (maximum in AMBIKA-II-I) mg GAE/100 g FW, and total flavonoids content ranged from 9.1 to 37.0 mg CE/100G FW (maximum in MWBS-16-26). In both DPPH and TEAC assay, hydrophilic antioxidant activity was towards higher side in AMBIKA-13-4 B (5.02 μmol TE/g FW and 3.06 μmol TE/g FW respectively). The genotypes AMBIKAWB-II-I, AMBIKA-13-4 B and MWBS-16-26 were identified superior based on their overall total phenolics, total flavonoids and antioxidant activities coupled with chemometric analysis.