Serial coupling of reversed‐phase and zwitterionic hydrophilic interaction <scp>LC</scp>/<scp>MS</scp> for the analysis of polar and nonpolar phenols in wine

Abstract

In the present study, an easy and efficient method based on the serial coupling of analytical reversed-phase and zwitterionic hydrophilic interaction liquid chromatography was developed for the simultaneous separation of polar and nonpolar phenols occurring in wine. The zwitterionic hydrophilic column was connected in series to the reversed-phase one via a T-piece, with which the ACN content in eluent of the second dimension was increased, in order to cope the solvent strength incompatibility between the two columns. The final mobile phase at low-flow rate (≤0.5 mL/min), high-ACN content (90%), and low-salt concentration was directed to an ESI-TOF-MS , for high accurate mass detections. The developed method was applied for the identification of target phenols in several wines. Retention time and peak width intra- and interday repeatability studies proved the reliability of the method for the simultaneous analysis of all the polar and nonpolar analytes in wine. The serial reversed-phase/zwitterionic hydrophilic interaction liquid chromatography coupling offered the possibility to enlarge the number of identified compounds and it represents a valid approach for nontarget analysis of complex samples by a single injection.