TOTAL PHENOLIC AND FLAVONOID CONTENTS AND ANTIOXIDANT PROPERTIES OF THAI TRADITIONAL HERBAL

Abstract

Objective: The study investigated for the several of solvent on merits Trigasornmas formula in term of phenolic, flavonoid contents and antioxidant activities. Methods: This research studied the effects of extraction solvents in water, ethyl acetate, methanol, dichloromethane, ethanol and hexane extracts of Trigasormmas formula . Total phenolic and total flavonoid content were evaluated according to the Folin- Ciocalteu procedure and a aluminium chloride colorimetric method, respectively. Two methods of antioxidant activities were used DPPH radical scavenging assay (DPPH assay) and Ferric Reducing Antioxidant Power (FRAP) assay. According to our study, the outcomes of free radical scavenging properties were demonstrated in terms of mg gallic acid equivalent (GAE)/100 g sample and mg ferric sulfate equivalent (FeSO 4 )/100 g sample antioxidant, respectively. Results: The average total phenolic content of water extract was 1,955.23±60.87 mg GAE/100 g sample which was higher than the other solvents while the methanol extract showed the highest flavonoid content at about 321.15±9.12 mg FeSO 4 /100g sample. For antioxidant properties, DPPH and FRAP assay, the highest values were found in water extract at about 158.93±12.45 mg GAE/100g sample and 2,118.87±24.38 mg FeSO 4 /100g sample, respectively. Conclusion: The obtained results support the use of this Thai traditional herbal formula, and suggest more investigation.