Protein Engineering

Protein engineering is a biotechnological field that designs and modifies proteins by altering their amino acid sequences to improve existing biological properties or generate novel functional capabilities.

43.5K

Publications

2.4M

Citations

145.4K

Authors

10.2K

Institutions

Publications per year

2017–2026

10.9K

Parents

Biomolecular EngineeringBiotechnologyChemical BiologyPharmaceutical Biotechnology

Children

Allosteric Protein EngineeringMembrane Protein EngineeringPost-translational Modification EngineeringProtein AggregationProtein CharacterizationProtein DesignProtein Expression OptimizationProtein GlycoengineeringProtein PurificationProtein Scaffold Engineering

Publications

43.5K

Protein production by auto-induction in high-density shaking cultures

F. William Studier · Protein Expression and Purification · 2005 · 6.1K citations

Life Sciences, Biochemistry, Biotechnology +12

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations

Life Sciences, Biochemistry, Molecular Biology +12

LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions

Andrew C. Wallace, Roman A. Laskowski, Janet M. Thornton · Protein Engineering Design and Selection · 1995 · 5.7K citations

Life Sciences, Biochemistry, Molecular Biology +14

Synthesis of Proteins by Native Chemical Ligation

Philip E. Dawson, Tom W. Muir, Ian Clark‐Lewis et al. · Science · 1994 · 4.1K citations

Life Sciences, Biochemistry, Molecular Biology +17

Filamentous Fusion Phage: Novel Expression Vectors That Display Cloned Antigens on the Virion Surface

George P. Smith · Science · 1985 · 4.1K citations

Life Sciences, Biotechnology, Molecular Biology +16

1–5 of 43.5K

Authors

145.4K

DB
David Baker
University of Washington, United States
138 publications · 19K citations
71
h-index
PG
Peter G. Schultz
Scripps Research Institute, United States
115 publications · 15.8K citations
68
h-index
AP
Andreas Plückthun
University of Zurich, Switzerland
121 publications · 13K citations
66
h-index
FH
Frances H. Arnold
California Institute of Technology, United States
75 publications · 9.2K citations
57
h-index
WF
William F. DeGrado
University of Pennsylvania, United States
59 publications · 7K citations
43
h-index

1–5 of 145.4K

Institutions

10.2K

1–8 of 10.2K

Venues

Proceedings of the National Academy of Sciences
PNAS · 1.1K publications · 139.1K citations
185
h-index
Journal of the American Chemical Society
JACS · 1.2K publications · 108.8K citations
155
h-index
Journal of Biological Chemistry
JBC · 1.6K publications · 114.4K citations
144
h-index
Science
194 publications · 70.9K citations
133
h-index
Angewandte Chemie International Edition
686 publications · 52.9K citations
117
h-index

1–5

See also Protein Engineering
Core Methods	Directed Evolution Rational Design Site-directed Mutagenesis Error-prone Pcr Site-saturation Mutagenesis Semi-rational Design Machine Learning-guided Protein Engineering Gene Shuffling De Novo Protein Design
Applications	Synthetic Biology Drug Discovery Biocatalysis Molecular Imaging Nanotechnology Biomaterials Biosensors Bispecific Antibodies Enzyme Replacement Therapy Diagnostics Plastic Recycling Industrial Chemistry Protein-based Vaccines Cancer Immunotherapy Protein-based Nanocarriers
Evaluation	Catalytic Activity Binding Affinity Substrate Selectivity Scoring Functions Thermostability Folding Free Energy Surface Solubility Fluorescence Spectroscopy
Variants	Enzymes Bispecific Antibodies Protein Binding Domains Antibodies Protein-based Vaccines Protein-based Nanocarriers
Limitations	Epistasis Activity Tradeoff