Concepedia

Site-directed Mutagenesis

Site-Directed Mutagenesis is a molecular biology technique in genetic engineering and recombinant DNA technology that introduces specific, predetermined nucleotide changes at defined sites within a DNA sequence, typically using designed oligonucleotide primers rather than random mutational processes.

2.9K

Publications

247.6K

Citations

10.4K

Authors

1.9K

Institutions

Publications per year

2017–2026

172

Variants

Sdm

Parents

Children

Publications

2.9K

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations

, , +10

Rapid and efficient site-specific mutagenesis without phenotypic selection.

Thomas A. Kunkel · Proceedings of the National Academy of Sciences · 1985 · 7.3K citations · Full text

, , +15

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

Reinhard Simon, U. B. Priefer, Alfred Pühler · Bio/Technology · 1983 · 7.3K citations

, , +12

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

Jeffrey Vieira, Joachim Messing · Gene · 1982 · 6.1K citations

, , +15

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

Thomas A. Kunkel, John D. Roberts, Richard A. Zakour · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 5.3K citations

, , +8

1–5 of 2.9K

Authors

10.4K

  1. BW
    Barry W. Glickman

    University of Victoria, Canada

    12 publications · 550 citations

    12

    h-index

  2. LA
    Lawrence A. Loeb

    University of Washington, United States

    13 publications · 2.5K citations

    12

    h-index

  3. RS
    R. Stephen Lloyd

    Vanderbilt University, United States

    10 publications · 391 citations

    10

    h-index

  4. MJ
    Michael J. Smith

    University of British Columbia, Canada

    10 publications · 3.9K citations

    10

    h-index

  5. PM
    Peter M. Glazer

    Yale University, United States

    10 publications · 1.1K citations

    10

    h-index

1–5 of 10.4K

Institutions

1.9K

  1. Harvard University

    Cambridge, United States

    42 h-index

    244 publications

    64.2K citations

  2. Centre National de la Recherche Scientifique

    Paris, France

    38 h-index

    253 publications

    14K citations

  3. National Institutes of Health

    Bethesda, United States

    32 h-index

    175 publications

    16.3K citations

  4. Howard Hughes Medical Institute

    Chevy Chase, United States

    32 h-index

    114 publications

    16.8K citations

  5. Massachusetts Institute of Technology

    Cambridge, United States

    30 h-index

    127 publications

    23.1K citations

  6. Yale University

    New Haven, United States

    29 h-index

    129 publications

    11.7K citations

  7. University of Washington

    Seattle, United States

    28 h-index

    102 publications

    12K citations

  8. University of Minnesota

    Minneapolis, United States

    27 h-index

    122 publications

    41.2K citations

1–8 of 1.9K

Venues

  1. Nucleic Acids Research

    173 publications · 18.8K citations

    60

    h-index

  2. Proceedings of the National Academy of Sciences

    PNAS · 91 publications · 18.8K citations

    57

    h-index

  3. Journal of Molecular Biology

    JMB · 132 publications · 9.8K citations

    56

    h-index

  4. Journal of Biological Chemistry

    JBC · 163 publications · 11K citations

    55

    h-index

  5. Nature

    53 publications · 10.5K citations

    46

    h-index

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