Error-Prone PCR is a site-directed mutagenesis method that introduces random mutations during DNA amplification by reducing the fidelity of the polymerase enzyme, thereby enabling the generation of diverse genotypes for functional exploration in directed evolution studies.
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‘Touchdown’ PCR to circumvent spurious priming during gene amplification
R.H. Don, Peter Cox, Brandon J. Wainwright et al. · Nucleic Acids Research · 1991 · 2.6K citations · Full text
Examining Sources of Error in PCR by Single-Molecule Sequencing
Vladimir Potapov, Jennifer L. Ong · PLoS ONE · 2017 · 340 citations · Full text
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Chinese Academy of Sciences
Beijing, China
10 h-index
42 publications
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University of Groningen
Groningen, The Netherlands
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29 publications
1.5K citations
Jiangnan University
Wuxi, China
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37 publications
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University of Chinese Academy of Sciences
Beijing, China
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Tianjin University
Tianjin, China
5 h-index
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590 citations
National Institutes of Health
Bethesda, United States
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Shanghai, China
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AEM · 14 publications · 1.6K citations
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PEDS · 14 publications · 530 citations
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