Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

Randall K. Saiki, David H. Gelfand, Susanne Stoffel et al. · Science · 1988 · 17.1K citations

Engineering, Dna Analysis, Molecular Biology +19

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

Randall K. Saiki, Stephen J. Scharf, Fred Faloona et al. · Science · 1985 · 9K citations

Immunohematology, Genetic Testing, Genetics +20

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

Kary B. Mullis, Fred Faloona · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 5.7K citations

Biosynthesis, Nucleic Acid Chemistry, Biochemistry +6

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

Robert M. Horton, Henry D. Hunt, Steffan N. Ho et al. · Gene · 1989 · 3.1K citations

Engineering, Hybridisation, Hybridization +13

A general method of<i>in vitro</i>preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

Russell Higuchi, Barbara Krummel, Randall K. Saiki · Nucleic Acids Research · 1988 · 2.4K citations · Full text