Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations

Natural Sciences, Genetics, Directed Evolution +6

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

Kary B. Mullis, Fred Faloona · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 5.7K citations

Biosynthesis, Nucleic Acid Chemistry, Biochemistry +6

Structure of the human class I histocompatibility antigen, HLA-A2

Pamela J. Björkman, Mark A. Saper, Boudjéma Samraoui et al. · Nature · 1987 · 3.5K citations

Hla, Histocompatibility, Human Class +7

A general method of<i>in vitro</i>preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

Russell Higuchi, Barbara Krummel, Randall K. Saiki · Nucleic Acids Research · 1988 · 2.4K citations · Full text

Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase.

Robert Kraft, Jil C. Tardiff, KS Krauter et al. · PubMed · 1988 · 558 citations

Dna Sequencing, Genetics, Mini-prep Plasmid Dna +8