Protein Expression and Purification

Protein production by auto-induction in high-density shaking cultures

F. William Studier · 2005 · 6.1K citations

Life Sciences, Biochemistry, Biotechnology +12

Purification of brain tubulin through two cycles of polymerization–depolymerization in a high-molarity buffer

M. Castoldi, A. V. Popov · 2003 · 777 citations

Life Sciences, Biochemistry, Neuroscience +8

Overcoming Expression and Purification Problems of RhoGDI Using a Family of “Parallel” Expression Vectors

Peter Sheffield, Sarah Garrard, Zygmunt S. Derewenda · 1999 · 658 citations

Life Sciences, Biochemistry, Molecular Biology +12

A New Vector for High-Throughput, Ligation-Independent Cloning Encoding a Tobacco Etch Virus Protease Cleavage Site

Lucy Stols, Minyi Gu, Lynda Dieckman et al. · 2002 · 543 citations

Life Sciences, Biotechnology, Molecular Biology +11

Comparison of affinity tags for protein purification

Jordan J. Lichty, Joshua L. Malecki, Heather D. Agnew et al. · 2005 · 408 citations

Life Sciences, Biochemistry, Molecular Biology +14