Concepedia

Protein Purification

Protein Purification is a biochemical engineering process within the broader field of bioseparations that specifically involves the isolation of a target protein from a complex biological mixture through selective separation techniques to achieve homogeneity while maintaining the protein’s native structure and biological activity.

23.4K

Publications

1.3M

Citations

64.9K

Authors

6.7K

Institutions

Publications per year

2017–2026

2.6K

Parents

Children

Publications

23.4K

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

Hermann Schägger, Gebhard von Jagow · Analytical Biochemistry · 1987 · 11.5K citations

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Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

Haim Aviv, Philip Leder · Proceedings of the National Academy of Sciences · 1972 · 6.9K citations

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Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations

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Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

Paul Matsudaira · Journal of Biological Chemistry · 1987 · 5K citations · Full text

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Quantitative analysis of complex protein mixtures using isotope-coded affinity tags

Steven P. Gygi, Beate Rist, Scott A. Gerber et al. · Nature Biotechnology · 1999 · 4.7K citations

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1–5 of 23.4K

Authors

64.9K

  1. RE
    Rotimi E. Aluko

    University of Manitoba, Canada

    40 publications · 4.4K citations

    33

    h-index

  2. LB
    Laurent Bazinet

    Université Laval, Canada

    40 publications · 1.9K citations

    28

    h-index

  3. AL
    Andrew L. Zydney

    Pennsylvania State University, United States

    39 publications · 2.3K citations

    28

    h-index

  4. PG
    Pier Giorgio Righetti

    University of Milan, Italy

    54 publications · 2.8K citations

    27

    h-index

  5. CE
    Charles E. Glatz

    Iowa State University, United States

    34 publications · 1.2K citations

    25

    h-index

1–5 of 64.9K

Institutions

6.7K

  1. National Institutes of Health

    Bethesda, United States

    83 h-index

    815 publications

    91.3K citations

  2. Harvard University

    Cambridge, United States

    78 h-index

    569 publications

    46.8K citations

  3. Centre National de la Recherche Scientifique

    Paris, France

    67 h-index

    1.1K publications

    51.6K citations

  4. University of Wisconsin–Madison

    Madison, United States

    58 h-index

    539 publications

    31.5K citations

  5. Wageningen University & Research

    Wageningen, The Netherlands

    54 h-index

    450 publications

    32.8K citations

  6. Kyoto University

    Kyoto, Japan

    53 h-index

    608 publications

    30.6K citations

  7. Cornell University

    Ithaca, United States

    53 h-index

    318 publications

    18.6K citations

  8. The University of Tokyo

    Tokyo, Japan

    53 h-index

    593 publications

    31.5K citations

1–8 of 6.7K

Venues

  1. Journal of Biological Chemistry

    JBC · 1.9K publications · 202.5K citations

    197

    h-index

  2. Proceedings of the National Academy of Sciences

    PNAS · 303 publications · 50.6K citations

    110

    h-index

  3. European Journal of Biochemistry

    EJB · 747 publications · 44.2K citations

    101

    h-index

  4. Biochemical Journal

    BJ · 630 publications · 34.3K citations

    93

    h-index

  5. Food Chemistry

    281 publications · 22.3K citations

    87

    h-index

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