Concepedia

Pam-dependent Specificity

PAM-Dependent Specificity is a targeting constraint in CRISPR-Cas systems that refers to the requirement for a specific protospacer adjacent motif (PAM) sequence adjacent to the target DNA site, thereby distinguishing it from PAM-independent mechanisms by restricting cleavage activity to genomic loci flanked by a predetermined nucleotide signature.

63

Publications

7.9K

Citations

419

Authors

162

Institutions

Publications per year

2017–2026

51

Parents

Publications

63

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

Johnny H. Hu, Shannon M. Miller, Maarten H. Geurts et al. · Nature · 2018 · 1.6K citations

, , +12

Engineered Cpf1 variants with altered PAM specificities

Linyi Gao, David Cox, Winston X. Yan et al. · Nature Biotechnology · 2017 · 470 citations · Full text

, , +6

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

Ryan T. Leenay, Kenneth R. Maksimchuk, Rebecca Slotkowski et al. · Molecular Cell · 2016 · 361 citations · Full text

, , +14

Protospacer recognition motifs

Shiraz A. Shah, Susanne Erdmann, Francisco J. M. Mojica et al. · RNA Biology · 2013 · 360 citations · Full text

, , +13

Continuous evolution of SpCas9 variants compatible with non-G PAMs

Shannon M. Miller, Tina Wang, Peyton B. Randolph et al. · Nature Biotechnology · 2020 · 357 citations · Full text

, , +8

1–5 of 63

Authors

419

  1. PC
    Pranam Chatterjee

    Duke University, United States

    4 publications · 509 citations

    4

    h-index

  2. ON
    Osamu Nureki

    The University of Tokyo, Japan

    4 publications · 1K citations

    4

    h-index

  3. JM
    Joseph M. Jacobson

    Massachusetts Institute of Technology, United States

    4 publications · 509 citations

    4

    h-index

  4. NJ
    Noah Jakimo

    Massachusetts Institute of Technology, United States

    4 publications · 509 citations

    4

    h-index

  5. HN
    Hiroshi Nishimasu

    The University of Tokyo, Japan

    4 publications · 1K citations

    4

    h-index

1–5 of 419

Institutions

162

  1. Massachusetts Institute of Technology

    Cambridge, United States

    8 h-index

    27 publications

    5.5K citations

  2. Harvard University

    Cambridge, United States

    7 h-index

    32 publications

    22.8K citations

  3. Broad Institute

    Cambridge, United States

    7 h-index

    35 publications

    24.9K citations

  4. Chinese Academy of Sciences

    Beijing, China

    5 h-index

    19 publications

    1.5K citations

  5. University of Chinese Academy of Sciences

    Beijing, China

    5 h-index

    14 publications

    1K citations

  6. The University of Tokyo

    Tokyo, Japan

    4 h-index

    15 publications

    3.7K citations

  7. Chinese Academy of Agricultural Sciences

    Beijing, China

    4 h-index

    29 publications

    2.1K citations

  8. MIT-Harvard Center for Ultracold Atoms

    Cambridge, United States

    4 h-index

    18 publications

    2.2K citations

1–8 of 162

Venues

  1. Nature Communications

    10 publications · 736 citations

    10

    h-index

  2. Molecular Cell

    6 publications · 1.2K citations

    6

    h-index

  3. Nature Biotechnology

    5 publications · 1.1K citations

    5

    h-index

  4. Nucleic Acids Research

    6 publications · 478 citations

    5

    h-index

  5. Plant Biotechnology Journal

    4 publications · 289 citations

    4

    h-index

1–5