Rationally engineered Cas9 nucleases with improved specificity

Ian M. Slaymaker, Linyi Gao, Bernd Zetsche, +3

Science

Engineered CRISPR-Cas9 nuclease with expanded targeting space

Hiroshi Nishimasu, Xi Shi, Soh Ishiguro, +18

Science

Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies

Paul W. Tillberg, Fei Chen, Kiryl D. Piatkevich, +15

Nature Biotechnology

Fluorescence MicroscopyImmunocytochemical TechniqueEngineeringTissue ImagingMicroscopy +10

Diverse enzymatic activities mediate antiviral immunity in prokaryotes

Linyi Gao, Han Altae-Tran, Francisca Böhning, +7

Science

Viral ReplicationEngineeringReverse GeneticsMolecular BiologyMicrobial Virus +19

Engineered Cpf1 variants with altered PAM specificities

Linyi Gao, David Cox, Winston X. Yan, +7

Nature Biotechnology

Engineered Cpf1 VariantsSignal TransductionEngineeringProtein AssemblyProtein Function +5

BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks

Winston X. Yan, Reza Mirzazadeh, Silvano Garnerone, +12

Nature Communications

Genome InstabilityDna SequencingDna Double-strand BreaksNatural SciencesGenetics +10

Engineering of CRISPR-Cas12b for human genome editing

Jonathan Strecker, Sara R. Jones, Balwina Koopal, +6

Nature Communications

Prokaryotic innate immunity through pattern recognition of conserved viral proteins

Linyi Gao, Max E. Wilkinson, Jonathan Strecker, +4

Science

Conserved Viral ProteinsPathogenesisImmunologyImmune Pattern-recognition ReceptorsMolecular Biology +13

Highly Parallel Profiling of Cas9 Variant Specificity

Jonathan L. Schmid‐Burgk, Linyi Gao, David Li, +4

Molecular Cell

Functional GenomicsMedicineGeneticsComputational GenomicsComputational Biology +5

Structural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1

Hiroshi Nishimasu, Takashi Yamano, Linyi Gao, +3

Molecular Cell

EngineeringBiomolecular ToolGenetic EngineeringMolecular BiologySynthetic Biology +8