Concepedia

Pam Specificity Engineering

PAM Specificity Engineering is a CRISPR-based engineering method that involves the directed modification of Cas nucleases to recognize altered or extended protospacer adjacent motifs, thereby expanding the targetable genomic space while maintaining editing precision.

49

Publications

11.2K

Citations

347

Authors

128

Institutions

Publications per year

2017–2026

41

Parents

Publications

49

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

Benjamin P. Kleinstiver, Michelle S. Prew, Shengdar Q. Tsai et al. · Nature · 2015 · 1.7K citations · Full text

, , +13

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

Johnny H. Hu, Shannon M. Miller, Maarten H. Geurts et al. · Nature · 2018 · 1.6K citations

, , +12

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

Carolin Anders, Ole Niewoehner, A. Duerst et al. · Nature · 2014 · 1.4K citations

, , +13

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

Russell T. Walton, Kathleen A. Christie, Madelynn N. Whittaker et al. · Science · 2020 · 1.3K citations · Full text

, , +15

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

Benjamin P. Kleinstiver, Michelle S. Prew, Shengdar Q. Tsai et al. · Nature Biotechnology · 2015 · 630 citations · Full text

, , +12

1–5 of 49

Authors

347

  1. PC
    Pranam Chatterjee

    Duke University, United States

    5 publications · 566 citations

    5

    h-index

  2. BP
    Benjamin P. Kleinstiver

    Massachusetts General Hospital, United States

    5 publications · 3.7K citations

    5

    h-index

  3. NJ
    Noah Jakimo

    Massachusetts Institute of Technology, United States

    5 publications · 566 citations

    5

    h-index

  4. HN
    Hiroshi Nishimasu

    The University of Tokyo, Japan

    5 publications · 955 citations

    5

    h-index

  5. ON
    Osamu Nureki

    The University of Tokyo, Japan

    5 publications · 955 citations

    5

    h-index

1–5 of 347

Institutions

128

  1. Harvard University

    Cambridge, United States

    10 h-index

    36 publications

    27.2K citations

  2. Massachusetts Institute of Technology

    Cambridge, United States

    7 h-index

    27 publications

    4.7K citations

  3. Broad Institute

    Cambridge, United States

    6 h-index

    28 publications

    22.3K citations

  4. Massachusetts General Hospital

    Boston, United States

    5 h-index

    28 publications

    29.1K citations

  5. The University of Tokyo

    Tokyo, Japan

    5 h-index

    16 publications

    2.9K citations

  6. University of Zurich

    Zurich, Switzerland

    4 h-index

    19 publications

    6.7K citations

  7. MIT-Harvard Center for Ultracold Atoms

    Cambridge, United States

    4 h-index

    18 publications

    2.2K citations

  8. Chinese Academy of Sciences

    Beijing, China

    4 h-index

    13 publications

    2.7K citations

1–8 of 128

Venues

  1. Nature Communications

    7 publications · 563 citations

    7

    h-index

  2. Nature Biotechnology

    5 publications · 1.7K citations

    5

    h-index

  3. Nucleic Acids Research

    4 publications · 351 citations

    4

    h-index

  4. Nature Chemical Biology

    3 publications · 94 citations

    3

    h-index

  5. Nature

    3 publications · 4.6K citations

    3

    h-index

1–5