Environmentally Friendly Extraction of Bioactive Compounds from Rosa canina L. fruits Using Deep Eutectic Solvent (DES) as Green Extraction Media

Abstract

In this study, the green extraction of Bioactive compounds and antioxidant activity from Rosehip (Rosa canina L.) fruits was investigated using ultrasound-assisted extraction combined with deep eutectic solvents (DES). A deep eutectic solvent-based on citric acid has been specially designed. Hydrogen bond donors (HBD) such as glycerol and ethylene glycol were used and a hydrogen bond acceptor (HBA) like citric acid was used. After choosing the best option of DES, extraction of the bioactive ingredients, optimal extraction conditions of the ultrasonic-assisted extraction have been optimized through the Box-Behnken design of response surface methodology (RSM). Total phenolics content (TPC), total anthocyanins content (TAA), and antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) have been found as 103.37 mg GAE/g DW in DES2, 92.23 mg GAE/g DW in DES1, 3.25mg C3G/100g-DW in DES2,1.31 mg C3G/100g-DW in DES1, and 101.85% Inhibition in DES2, 94.32 %. The results showed that this method is a competitive sustainable, green, and effective extraction of bioactive compounds from Rosehip (Rosa canina L.) fruits.