Gas Chromatography–Mass Spectrometry (GC-MS/MS) Analysis, Ultrasonic Assisted Extraction, Antibacterial and Antifungal Activity of Emblica officinalis Fruit Extract

Abstract

Introduction: Emblica officinalis is one of the common plants being used traditionally in different ways to search for cures and relief from various diseases. Among these diseases, Emblica officinalis had many of medicinal uses in treating a wide variety of bacterial and fungal infections. In this study, Ultrasonic assisted extraction (UAE) was efficiently used for the preparation of aqueous and methanolic extracts of Embelica officinalis fruits (EOFE). The phytoconstituents was detected through GC-MS/MS analysis to confirm antimicrobial effect of EOFE. Materials and Methods: The extraction was carried out at 20C, for 20 min using a solid-to-solvent ratio of 1: 60 w/v. The extracts were subjected to GC-MS/MS analysis. The in-vitro effect of extracts against twenty one microbial strains was investigated by an agar well diffusion method in different concentrations (25 g/mL-1000 g/mL). Results: