Multiplex Genome Engineering Using CRISPR/Cas Systems

Le Cong, F. Ann Ran, David Cox, +8

Science

Single Crispr ArrayEngineeringShort RnasGeneticsSynthetic Biology +10

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

John G. Doench, Nicolò Fusi, Meagan E. Sullender, +10

Nature Biotechnology

Systems BiologyOff-target EffectNatural SciencesMolecular BiologySgrna Design +8

Simultaneous epitope and transcriptome measurement in single cells

Marlon Stoeckius, Christoph Hafemeister, William Stephenson, +5

Nature Methods

Systems BiologySingle Cell SequencingMedicineOmicsCell Biology +6

Mutations Associated with Acquired Resistance to PD-1 Blockade in Melanoma

Jesse M. Zaretsky, Ángel García-Díaz, Daniel Sanghoon Shin, +26

New England Journal of Medicine

An immunogenic personal neoantigen vaccine for patients with melanoma

Patrick A. Ott, Zhuting Hu, Derin B. Keskin, +37

Nature

VaccinationTumor ImmunologyMelanomaImmunologyTherapeutic Vaccine +3

Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum

Sean C. Bendall, Erin F. Simonds, Peng Qiu, +13

Science

Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry

Charlotte Giesen, Hao A. O. Wang, Denis Schapiro, +10

Nature Methods

Subcellular ResolutionTissue ImagingOncologic ImagingMedical ImagingMicroscopy +10

Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens

Atray Dixit, Oren Parnas, Biyu Li, +13

Cell

Functional GenomicsPooled Genetic ScreensMedicineGeneticsSingle Cell Sequencing +12

viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia

El-ad David Amir, Kara L. Davis, Michelle D. Tadmor, +7

Nature Biotechnology

EngineeringCell DivisionSingle Cell SequencingMedicineSpatial Omics +8

Loss of IFN-γ Pathway Genes in Tumor Cells as a Mechanism of Resistance to Anti-CTLA-4 Therapy

Jianjun Gao, Lewis Z. Shi, Hao Zhao, +13

Cell

Tumor BiologyCancer ImmunosurveillanceMedicineIfn-γ Pathway GenesImmunology +8