Isolation and characterization of bacteriocin producing Enterococcus casseliflavus and its antagonistic effect on Pseudomonas aeruginosa

Abstract

The discovery of antimicrobial bacteriocin molecules with broad-spectrum activity against bacterial infections caused by spoilage microorganisms is vital for food safety. In this study, a potential bacteriocin-producing bacterium was isolated, screened and confirmed as Enterococcus casseliflavus MI001 by 16s rDNA sequencing. The bacteriocin peptide was purified and analysed by MALDI-TOF, which revealed the presence of a bacteriocin ABC transporter protein with a molecular mass of 22.53 kDa. The purified bacteriocin gave a final yield of 2.0%, with a specific activity of 15,000 AU/mg. The bacteriocin showed antagonistic activity against Pseudomonas aeruginosa and has potential as an antioxidant.