Determination of aflatoxins in medicinal herbs by HPLC. An efficient method for routine analysis

Abstract

A fast and easy to perform method for the routine determination of aflatoxins in medicinal herbs was developed. The described method involves a single-step extraction with a non-chlorinated solvent, an immunoaffinity clean-up and HPLC with fluorescence detection. Whilst assays with naturally contaminated and with spiked samples of several herbs showed that the recoveries were somewhat low and dependent on the kind of sample and the degree of grinding, the intra-batch reproducibility was good, allowing a reliable quantitation by the standard-addition method. Good linearity, repeatability and accuracy were demonstrated in assays involving several medicinal herbs. The limit of quantitation was of the order of 0.05-0.1 ng/g, being dependent of the species analysed, and the method required no tedious concentration or back-extraction steps.