Purification of an ACE Inhibitory Peptide from Hydrolysates of Duck Meat Protein

Abstract

An angiotensin converting enzyme (ACE) inhibitory peptide was isolated, purified from the hydrolysates of duck meat protein. Duck meat protein was hydrolyzed using trypsin at 37℃ for 2 hrs. An ACE inhibitory peptide was purified using membrane filtration, anion exchange chromatography, gel permeation chromatography, fast protein liquid chromatography, normal phase HPLC. The purified inhibitory peptide was identified to be a tetrapeptide, Glu-Asp-Leu-Glu having IC_(50) value of 85.9 μM.