Journal of Medicinal Food · 2007 · 121 citations · 25 references
Natural SciencesBiochemistryMarine SciencesFood AnalysisFood ChemistryGreen ChemistryAntioxidant DefenseFood AntioxidantsMarine Bioactive CompoundsMarine Natural ProductsSeaweed CultureAntioxidant InhibitionAntioxidant PropertiesExtractFree Radical ScavengingMarine Phenolic CompoundsPhenolic CompoundsScavengingThiobarbituric Acid Reactive Substances
Antioxidant properties of brown seaweed (Sargassum siliquastrum) extracts were evaluated using various antioxidant measurements, i.e., inhibitory effect on thiobarbituric acid-reactive substances (TBARS), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, metal chelating effect, reducing power effect, and total phenolic compounds. When the extraction solvents n-hexane, chloroform, ethanol, and water were compared, the water extract showed the highest yield in extracted mass. Total phenolic compounds were the highest in the ethanol extract, with 127.4 mg/g. The TBARS inhibition of chloroform and ethanol extracts at 10 mg/mL was 90.9% and 80.9%, respectively. DPPH radical scavenging capacity was more than 90% in all extracts at 1 mg/mL. The chloroform extract exhibited the highest metal ion chelating ability of 69.6% at 10 mg/mL. The reducing power was found to be the highest in the ethanol extract at 10 mg/mL, showing an effect similar to ascorbic acid. Thus, the ethanol extract of S. siliquastrum has potential as a natural antioxidant.
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Antioxidant Determinations by the Use of a Stable Free Radical
Marsden S. Blois · Nature · 1958 · 12.8K citations