Triacylglycerols-Profiling by High Performance Liquid Chromatography: A Tool for Detection of Pork Fat (Lard) in Processed Foods

Abstract

Abstract A rapid high-performance liquid chromatographic procedure for separation of the triacylglycerols (TG) of animal fats using refractive index detection is described. A LiChrospher-100 RP-18 (5 μm) column was used for the TG-profiling of pork, beef, mutton, chicken and turkey fats. Detection of pork fat in processed foods and lard in fat-admixtures is also discussed. TG-separation and checking genuinity and adulteration was achieved isocratically in ∼ 15 min. by using CH3CN/CH2Cl2 (58:42, v/v) at ambient temperature.