Journal of Food Science · 2006 · 47 citations · 36 references
PharmacyBiochemistryFood ScienceCarbohydrate BiochemistryCarbohydrate ChemistryFood ChemistryCarbohydrate AnalysisCarbohydrate CharacterizationCarbohydrates In FoodsGlycan AnalysisProtein AnalysisStarch ChemistryAcid-catalyzed HydrolysisHydrolysisStarch Chain-length DistributionStarch Fine StructureStarch HydrolysisStarch Physicochemical PropertiesStarch StructureSweet Potato
ABSTRACT: Fine structure of sweet potato starch (SPS) and mung bean starch (MBS) by gel‐permeation chromatography (GPC) showed that the amylose in SPS and MBS had 9.0 and 1.8 chains, respectively. The long chains of amylopectin in MBS (A p ‐MB) were longer than those of amylopectin in SPS (A p ‐SP), but the short chains of A p ‐SP were shorter than those of A p ‐MB. The structures of starch noodles of sweet potato (SPSN) and mung bean (MBSN) were analyzed by GPC, scanning electron micrograph (SEM), differential scanning calorimetry (DSC), and X‐ray diffraction after hydrolysis by acid and enzymes. The results showed that the residues obtained with acid and enzymes in MBSN contained large amounts of high molecular weight fractions, and a relatively small amount of low molecular weight fractions, whereas those in SPSN contained some high molecular weight fractions and large amounts of low molecular weight fractions. SPSN exhibited higher digestibility by HCl, α‐amylase, β‐amylase, and pullulanase than MBSN. The surface of MBSN was more smooth than that of SPSN and the inside of MBSN contained long, thick, and orderly filaments, while there were many pore spaces inside SPSN from SEM. The DSC thermogram of the resistant residues from both starch noodles after acid/enzyme hydrolysis showed a broad endotherm peak near 100 °C (96 to 115 °C) due to the presence of the complexes of amylose‐lipid and lipid‐(long chains in amylopectin). Because of a lower content of branched amylose and a higher content of amylopectin in SPS, the structure of SPSN had a less distinct crystalline pattern and higher adhesiveness, whereas there was a higher content of amylose with a little branch and moderate amylopectin in MBS. Thus, the structure of MBSN had a stronger distinct crystalline pattern and good cohesiveness.
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Colorimetric Method for Determination of Sugars and Related Substances
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Multi-branched nature of amylose and the action of debranching enzymes
Susumu Hizukuri, Yasuhito Takeda, Michiko Yasuda et al. · Carbohydrate Research · 1981 · 488 citations