A Triple-Mismatch Differentiating assay exploiting activation and trans cleavage of CRISPR-Cas12a for mutation detection with ultra specificity and sensitivity

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Bernd Zetsche, Jonathan S. Gootenberg, Omar O. Abudayyeh et al. · Cell · 2015 · 4.9K citations · Full text

Life Sciences, Genetics, Molecular Biology +12

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen, Enbo Ma, Lucas B. Harrington et al. · Science · 2018 · 4.4K citations

Life Sciences, Genetics, Molecular Biology +13

Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing

Benjamin P. Kleinstiver, Alexander A. Sousa, Russell T. Walton et al. · Nature Biotechnology · 2019 · 724 citations

Life Sciences, Biotechnology, Genetics +13

Functionally diverse type V CRISPR-Cas systems

Winston X. Yan, Pratyusha Hunnewell, Lauren E. Alfonse et al. · Science · 2018 · 524 citations · Full text

Life Sciences, Genetics, Molecular Biology +11

Increasing the specificity of CRISPR systems with engineered RNA secondary structures

D. Dewran Koçak, Eric A. Josephs, Vidit Bhandarkar et al. · Nature Biotechnology · 2019 · 361 citations

Life Sciences, Genetics, Molecular Biology +14