CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen, Enbo Ma, Lucas B. Harrington, +4

Science

Ssdna MoleculesMolecular Diagnostic TechniquesEngineeringMedicineSynthetic Biology +12

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

Janice S. Chen, Yavuz S. Dagdas, Benjamin P. Kleinstiver, +6

Nature

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

Lucas B. Harrington, David Burstein, Janice S. Chen, +7

Science

Programmed Dna DestructionOff-target EffectEngineeringAmino AcidsBiomolecular Tool +13

New CRISPR–Cas systems from uncultivated microbes

David Burstein, Lucas B. Harrington, Steven Strutt, +5

Nature

EngineeringBiotechnologySynthetic BiologyMolecular BiologyGenetic Engineering +7

CasX enzymes comprise a distinct family of RNA-guided genome editors

Junjie Liu, N. S. Orlova, Benjamin L. Oakes, +15

Nature

Distinct FamilyNatural SciencesRna BiologyMolecular BiologyDna Replication +7

A Broad-Spectrum Inhibitor of CRISPR-Cas9

Lucas B. Harrington, Kevin Doxzen, Enbo Ma, +12

Cell

Broad-spectrum InhibitorMolecular BiologyGenetic EngineeringGene EditingGenome Editing +3

A thermostable Cas9 with increased lifetime in human plasma

Lucas B. Harrington, David Páez-Espino, Brett T. Staahl, +4

Nature Communications

Thermostable Cas9EngineeringNonthermal PlasmaCas9 ProteinImmunology +12

Foreign DNA capture during CRISPR–Cas adaptive immunity

James K. Nuñez, Lucas B. Harrington, Philip J. Kranzusch, +2

Nature

ImmunologyGene EditingCrisprMedicineViral Immunity +2

CRISPR Immunological Memory Requires a Host Factor for Specificity

James K. Nuñez, Lawrence Bai, Lucas B. Harrington, +2

Molecular Cell

Immunological MemoryCrispr Immunological MemoryImmunologyGenetic EngineeringGene Editing +4

Single-Stranded DNA Cleavage by Divergent CRISPR-Cas9 Enzymes

Enbo Ma, Lucas B. Harrington, Mitchell R. O’Connell, +2

Molecular Cell

GeneticsMolecular BiologyGenetic EngineeringDivergent Crispr-cas9 EnzymesGene Editing +3