Search-and-replace genome editing without double-strand breaks or donor DNA

Andrew V. Anzalone, Peyton B. Randolph, Jessie R. Davis, +8

Nature

Genome SurgeryMedicineGeneticsMolecular BiologyGenetic Engineering +3

High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

R. Daniel Gietz, Robert H. Schiestl

Nature Protocols

Molecular BiotechnologyEngineeringNatural SciencesBiotechnologyDna Replication +4

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

Michelle F. Richter, Kevin T. Zhao, Elliot O. Eton, +9

Nature Biotechnology

EngineeringBiochemistryNatural SciencesPhage-assisted EvolutionPeptide Library +7

Effect of Genome Size on AAV Vector Packaging

Zhijian Wu, Hongyan Yang, Peter Colosi

Molecular Therapy

Viral ReplicationAav Vector GenomesVector GenomesGeneticsMolecular Biology +15

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

Peter J. Chen, Jeffrey A. Hussmann, Jun Yan, +11

Cell

Off-target EffectEngineeringPrime EditingMedicineGenetics +12

A system for the continuous directed evolution of biomolecules

Kevin M. Esvelt, Jacob C. Carlson, David R. Liu

Nature

Pattern FormationEngineeringDirected EvolutionComputational BiologyProtein Evolution +5

Engineered pegRNAs improve prime editing efficiency

James W. Nelson, Peyton B. Randolph, Simon P. Shen, +8

Nature Biotechnology

Natural SciencesMolecular BiologyGene EditingSmall RnaGene Expression +3

Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area

Sebastian Kügler, Mathias Bähr

Gene Therapy

NeuroregenerationSignal TransductionMolecular NeuroscienceAdenoviral VectorSynaptic Transmission +11

Scalable, Continuous Evolution of Genes at Mutation Rates above Genomic Error Thresholds

Arjun Ravikumar, Garri A. Arzumanyan, Muaeen K.A. Obadi, +2

Cell

Continuous EvolutionMutation-based TestingEvolution StrategyMedicineGenetics +9

Phage-assisted evolution and protein engineering yield compact, efficient prime editors

Jordan L. Doman, Smriti Pandey, Monica E. Neugebauer, +14

Cell