Concepedia

Directed Protein Evolution

Directed Protein Evolution is a laboratory method in stability engineering that involves the iterative application of mutagenesis and selection to engineer proteins with enhanced or novel functional properties, distinguished from natural protein evolution by its purposeful design and controlled experimental conditions.

103

Publications

5.9K

Citations

486

Authors

179

Institutions

Publications per year

2017–2026

31

Variants

In Vitro EvolutionLaboratory Evolution

Parents

Publications

103

Machine learning-assisted directed protein evolution with combinatorial libraries

Zachary Wu, S. B. Jennifer Kan, Russell D. Lewis et al. · Proceedings of the National Academy of Sciences · 2019 · 619 citations · Full text

, , +17

Picoliter Cell Lysate Assays in Microfluidic Droplet Compartments for Directed Enzyme Evolution

Bálint Kintses, Christopher Hein, Mark F. Mohamed et al. · Chemistry & Biology · 2012 · 232 citations

, , +13

Informed training set design enables efficient machine learning-assisted directed protein evolution

Bruce J. Wittmann, Yisong Yue, Frances H. Arnold · Cell Systems · 2021 · 218 citations · Full text

, , +12

Computational method to reduce the search space for directed protein evolution

Christopher A. Voigt, Stephen L. Mayo, Frances H. Arnold et al. · Proceedings of the National Academy of Sciences · 2001 · 179 citations · Full text

, , +16

Directed evolution of ligand dependence: Small-molecule-activated protein splicing

Allen R. Buskirk, Y. C. Ong, Zev J. Gartner et al. · Proceedings of the National Academy of Sciences · 2004 · 168 citations · Full text

, , +15

1–5 of 103

Authors

486

  1. US
    Ulrich Schwaneberg

    RWTH Aachen University, Germany

    7 publications · 406 citations

    7

    h-index

  2. FH
    Frances H. Arnold

    California Institute of Technology, United States

    4 publications · 1K citations

    4

    h-index

  3. IM
    Ichiro Matsumura

    Emory University, United States

    4 publications · 119 citations

    4

    h-index

  4. MA
    Miguel Alcalde

    Instituto de Catálisis y Petroleoquímica, Spain

    4 publications · 247 citations

    4

    h-index

  5. PT
    Petra Tafelmeyer

    Hybrigenics (France), France

    3 publications · 103 citations

    3

    h-index

1–5 of 486

Institutions

179

  1. California Institute of Technology

    Pasadena, United States

    7 h-index

    20 publications

    4.8K citations

  2. Harvard University

    Cambridge, United States

    6 h-index

    22 publications

    2.5K citations

  3. Constructor University

    Bremen, Germany

    6 h-index

    19 publications

    1.3K citations

  4. Emory University

    Atlanta, United States

    5 h-index

    9 publications

    374 citations

  5. Instituto de Catálisis y Petroleoquímica

    Madrid, Spain

    4 h-index

    17 publications

    995 citations

  6. RWTH Aachen University

    Aachen, Germany

    4 h-index

    11 publications

    262 citations

  7. University of Cambridge

    Cambridge, United Kingdom

    4 h-index

    16 publications

    2.3K citations

  8. University of Münster

    Münster, Germany

    4 h-index

    11 publications

    599 citations

1–8 of 179

Venues

  1. Chemistry & Biology

    5 publications · 472 citations

    5

    h-index

  2. Scientific Reports

    5 publications · 150 citations

    5

    h-index

  3. Protein Engineering Design and Selection

    PEDS · 4 publications · 188 citations

    4

    h-index

  4. Nature Communications

    4 publications · 164 citations

    4

    h-index

  5. Journal of the American Chemical Society

    JACS · 3 publications · 320 citations

    3

    h-index

1–5