Concepedia

Directed Evolution of Enantioselective Enzymes: Iterative Cycles of CASTing for Probing Protein‐Sequence Space

Manfred T. Reetz, Marco Bocola

Angewandte Chemie · 2006 · 115 citations · 74 references

DOI

Concepts

Abstract

Umbaumaßnahmen am Enzym: Der jüngst eingeführte CAST (=combinatorial active-site saturation test) wurde in Iterationszyklen bei der gerichteten Evolution enantioselektiver Wildtyp-Epoxidhydrolasen (WT-EH) angewendet.

References

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Combinatorial and Evolution-Based Methods in the Creation of Enantioselective Catalysts

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Expanding the Range of Substrate Acceptance of Enzymes: Combinatorial Active‐Site Saturation Test

Manfred T. Reetz, Marco Bocola, José Daniel Carballeira et al. · Angewandte Chemie International Edition · 2005 · 504 citations

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