Publication | Closed Access
Directed Evolution of Enantioselective Enzymes: Iterative Cycles of CASTing for Probing Protein‐Sequence Space
115
Citations
74
References
2006
Year
EngineeringBiochemistryNatural SciencesProtein‐sequence SpaceEnzyme CatalysisDirected EvolutionBiotechnologyMolecular BiologySynthetic BiologyIterative CyclesEnzyme SpecificityProtein EngineeringStructure-function Enzyme KineticsEnzymatic ModificationRedox Biology
Umbaumaßnahmen am Enzym: Der jüngst eingeführte CAST (=combinatorial active-site saturation test) wurde in Iterationszyklen bei der gerichteten Evolution enantioselektiver Wildtyp-Epoxidhydrolasen (WT-EH) angewendet.
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