Concepedia

Directed Evolution Of Active Sites

Directed Evolution of Active Sites is a methodological approach in protein engineering that involves the iterative application of mutagenesis and selection to optimize the catalytic residues and binding architecture of an enzyme's active site for enhanced or novel biochemical function.

26

Publications

2K

Citations

90

Authors

46

Institutions

Publications per year

2017–2026

8

Parents

Publications

26

Iterative Saturation Mutagenesis on the Basis of B Factors as a Strategy for Increasing Protein Thermostability

Manfred T. Reetz, José Daniel Carballeira, Andreas Vogel · Angewandte Chemie International Edition · 2006 · 484 citations

, , +17

Directed Evolution of Enantioselective Enzymes: Iterative Cycles of CASTing for Probing Protein‐Sequence Space

Manfred T. Reetz, Marco Bocola · Angewandte Chemie International Edition · 2006 · 362 citations

, , +17

Enzymatic Neutralization of the Chemical Warfare Agent VX: Evolution of Phosphotriesterase for Phosphorothiolate Hydrolysis

Andrew N. Bigley, Chengfu Xu, Terry J. Henderson et al. · Journal of the American Chemical Society · 2013 · 125 citations · Full text

, , +17

Optimising enzyme function by directed evolution

Paul A. Dalby · Current Opinion in Structural Biology · 2003 · 117 citations

, , +13

Directed Evolution of Enantioselective Enzymes: Iterative Cycles of CASTing for Probing Protein‐Sequence Space

Manfred T. Reetz, Marco Bocola · Angewandte Chemie · 2006 · 115 citations

, , +17

1–5 of 26

Authors

90

  1. MT
    Manfred T. Reetz

    Max-Planck-Institut für Kohlenforschung, Germany

    4 publications · 1.1K citations

    4

    h-index

  2. MB
    Marco Bocola

    RWTH Aachen University, Germany

    4 publications · 517 citations

    4

    h-index

  3. MT
    Manfred T. Reetz

    Max-Planck-Institut für Kohlenforschung, Germany

    2 publications · 40 citations

    2

    h-index

  4. LW
    Li‐Wen Wang

    Max-Planck-Institut für Kohlenforschung, Germany

    2 publications · 40 citations

    2

    h-index

  5. AV
    Andreas Vogel

    c-LEcta (Germany), Germany

    2 publications · 589 citations

    2

    h-index

1–5 of 90

Institutions

46

  1. Max-Planck-Institut für Kohlenforschung

    Mülheim, Germany

    6 h-index

    18 publications

    3.3K citations

  2. Biochemical Society

    London, United Kingdom

    2 h-index

    4 publications

    246 citations

  3. University College London

    London, United Kingdom

    2 h-index

    4 publications

    246 citations

  4. Max Planck Society

    Munich, Germany

    2 h-index

    6 publications

    1.5K citations

  5. University of California, Los Angeles

    Los Angeles, United States

    2 h-index

    13 publications

    890 citations

  6. Tata Institute of Fundamental Research

    Mumbai, India

    2 h-index

    3 publications

    115 citations

  7. Uppsala University

    Uppsala, Sweden

    1 h-index

    2 publications

    90 citations

  8. The University of Osaka

    Osaka, Japan

    1 h-index

    2 publications

    76 citations

1–8 of 46

Venues

  1. Angewandte Chemie International Edition

    3 publications · 868 citations

    3

    h-index

  2. Angewandte Chemie

    3 publications · 238 citations

    3

    h-index

  3. Journal of Molecular Biology

    JMB · 2 publications · 57 citations

    2

    h-index

  4. Journal of the American Chemical Society

    JACS · 2 publications · 145 citations

    2

    h-index

  5. PLoS ONE

    2 publications · 73 citations

    2

    h-index

1–5