Angewandte Chemie International Edition · 2006 · 362 citations · 76 references
Life SciencesBiochemistryBiotechnologyBiocatalysisDirected EvolutionEnzymologyDirected Evolution Of EnzymesEnzymatic CatalysisEnzyme Active SitesEnzyme DesignEnzyme DiscoveryEnzyme EvolutionActive Site EvolutionDirected Enzyme EvolutionDirected Evolution For EnantioselectivityDirected Evolution Of Active SitesEnantioselective BiocatalysisEnantioselective SynthesisEnzymatic SynthesisEnzyme Evolution Modeling
ReCASTing for success: The recently introduced method of combinatorial active-site saturation test (CAST) has been applied in iterative cycles in the directed evolution of enantioselective wild-type epoxide hydrolases (WT-EH).
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Philippe Métézeau · Research in Microbiology · 1998 · 1.3K citations
Randomization of genes by PCR mutagenesis.
R C Cadwell, Gerald F. Joyce · Genome Research · 1992 · 1.1K citations · Full text