Effect of extraction solvent on total polyphenol content, total flavonoid content, and antioxidant activity of soursop seeds (Annona muricata L.)

Abstract

Abstract Soursop ( Annona muricata L.), a plant native to South America, is widely distributed in tropical and subtropical regions of the world. In this study, extract of A. Muricata was obtained using various solvents including distilled water, ethanol, ethyl acetate, and chloroform. Total polyphenol content, total flavonoid content and the antioxidant activity of A. muricata seed extracts were investigated using various in vitro assays. The highest extraction yield (23.60%) was obtained by using chloroform. The extract obtained by ethanol showed the highest total polyphenol content (282.71 ± 8.64 mg GAE/100g DW) and the highest flavonoid content (86.57 ± 3.20 mg QE/100g DW). The same extract also exhibited the highest DPPH (341.57 ± 6.90 AAE/100g DW), ABTS (382.20 ± 9.71 mg AAE/100g DW) radical scavenging activity and FRAP (369.84 ± 7.96 mg AAE/100g DW). These results indicate that A. muricata can be used in dietary applications with the potential to reduce oxidative stress.