In vitro assessment of metabolic profile of Enterococcus strains of human origin

Abstract

In the present study, previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al., Asian J Pharm Clin Res 12: 2019). Thirteen enterococci strains of human origin produced important enzymes like amylase (0.5–0.7 mg ml −1 ), protease (192–264 mg ml −1 ), lipase (8–10 mg ml −1 ), bile salt hydrolase, conjugated linoleic acid (CLA), and lactic acid (highest 12 mg ml −1 ), thus implicating potential attributes of starter cultures in food and dairy industry. Biogenic amines like arginine and tryptamine were produced after 4 days above 25 °C. Castor oil (highest yield 60 μg ml −1 ) and sunflower oil (highest yield 48 μg ml −1 ) both proved to be excellent sources of CLA production. Reduction assays using FRAP, ABTS (above 83%), and DPPH (30–50%) revealed excellent radical scavenging properties of cell-free supernatants of Enterococcus strains. The results implicate the future potential of application enterococci for therapeutic purpose as well as the food industry.