Natural Product Research · 2019 · 27 citations · 5 references
Life SciencesBotanyPlant SciencesPlant BiochemistryPlant Molecular BiologyStructure-activity RelationshipsAntioxidant DefenseChemical Defense In PlantsMedicinal PlantsOxidative Damage To BiomoleculesPhenolic Compound AnalysisReactive Oxygen SpeciesAntioxidant PropertiesChemotaxonomic CharacterizationChemotaxonomic IdentificationOxidative Dna DamagePhenolic CompoundsPhytochemical CompositionPlant Antioxidant DefensePlant Extract Antioxidants
Ikonnikovia kaufmanniana is an endemic plant of Kazakhstan of which phytochemical analysis has not been reported. The present study found out that this species enriched with antioxidant chemicals. Isolation and structural identification processes reveal twelve phenolic compounds (1–12) having dihydroflavanonol, flavonol, isoflavone and flavanol skeletons. The annotation of individual components in the extract was carried out by LC-ESI-MS/MS to represent a chemotaxonomic marker of the target plant. The antioxidant activities of all compounds were screened using three different radical sources (DPPH, ORAC, and hydroxyl radicals). Most compounds (1–11) had significant antioxidant activity against three radical sources, and their efficacies were found to differ by their functionality and skeleton. The potential of the isolated compounds in preventing oxidative damage of DNA was evaluated with pBR322 plasmid DNA. Compounds (1, 5, 7, and 8) had protective effects on DNA damaged with 80% efficacy at 60 μM concentration.
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Use of ORAC to assess antioxidant capacity of human milk
A. Sáenz, Ingrid Elisia, Sheila M. Innis et al. · Journal of Food Composition and Analysis · 2009 · 31 citations