A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jínek, Krzysztof Chylinski, Ines Fonfara, +3

Science

Crispr RnasAdaptive Bacterial ImmunityCrispr-associated Protein Cas9MedicineGenetics +13

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

Nicole M. Gaudelli, Alexis C. Komor, Holly A. Rees, +4

Nature

Nucleic acid detection with CRISPR-Cas13a/C2c2

Jonathan S. Gootenberg, Omar O. Abudayyeh, Jeong Wook Lee, +16

Science

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

Yanfang Fu, Jennifer A Foden, Cyd Khayter, +4

Nature Biotechnology

In vivo genome editing using Staphylococcus aureus Cas9

F. Ann Ran, Le Cong, Winston X. Yan, +10

Nature

Genetic EngineeringGene EditingGenome EngineeringGenome EditingMicrobiology +3

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

Benjamin P. Kleinstiver, Vikram Pattanayak, Michelle S. Prew, +4

Nature

Natural SciencesGenetic EngineeringMolecular BiologyGenome EngineeringGene Editing +5

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

Elitza Deltcheva, Krzysztof Chylinski, Cynthia M. Sharma, +6

Nature

Natural SciencesGeneticsCrispr Rna MaturationRna BiologyMolecular Biology +7

Rationally engineered Cas9 nucleases with improved specificity

Ian M. Slaymaker, Linyi Gao, Bernd Zetsche, +3

Science

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Shengdar Q. Tsai, Zongli Zheng, Nathalie T. Nguyen, +9

Nature Biotechnology

Off-target EffectGeneticsGene EditingGenome EditingGenomics +5

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

Samuel H. Sternberg, Sy Redding, Martin Jínek, +2

Nature

Genetic EngineeringMolecular BiologyGene EditingGenome EditingMedicine +2