High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

Yanfang Fu, Jennifer A Foden, Cyd Khayter, +4

Nature Biotechnology

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Shengdar Q. Tsai, Zongli Zheng, Nathalie T. Nguyen, +9

Nature Biotechnology

Off-target EffectGeneticsGene EditingGenome EditingGenomics +5

FLASH assembly of TALENs for high-throughput genome editing

Deepak Reyon, Shengdar Q. Tsai, Cyd Khayter, +3

Nature Biotechnology

EngineeringGenome SurgerySynthetic BiologyGenetic EngineeringMolecular Biology +6

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

Shengdar Q. Tsai, Nicolas Wyvekens, Cyd Khayter, +6

Nature Biotechnology

Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA)

Jeffry D. Sander, Elizabeth J. Dahlborg, Mathew J. Goodwin, +20

Nature Methods

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In Situ Genetic Correction of the Sickle Cell Anemia Mutation in Human Induced Pluripotent Stem Cells Using Engineered Zinc Finger Nucleases

Vittorio Sebastiano, Morgan L. Maeder, James Angstman, +10

Stem Cells

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Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs

Lindsay Cade, Deepak Reyon, Woong Y. Hwang, +7

Nucleic Acids Research

Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs)

Finola E. Moore, Deepak Reyon, Jeffry D. Sander, +6

PLoS ONE

GeneticsGenomic MechanismMolecular BiologyCoda ZfnsEpigenetics +14

Dimeric CRISPR RNA-Guided FokI-dCas9 Nucleases Directed by Truncated gRNAs for Highly Specific Genome Editing

Nicolas Wyvekens, Ved V. Topkar, Cyd Khayter, +2

Human Gene Therapy

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Engineering Designer Transcription Activator‐‐Like Effector Nucleases (TALENs) by REAL or REAL‐Fast Assembly

Deepak Reyon, Cyd Khayter, Maureen Regan, +2

Current Protocols in Molecular Biology