High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

Benjamin P. Kleinstiver, Vikram Pattanayak, Michelle S. Prew, +4

Nature

Natural SciencesGenetic EngineeringMolecular BiologyGenome EngineeringGene Editing +5

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

Shengdar Q. Tsai, Zongli Zheng, Nathalie T. Nguyen, +9

Nature Biotechnology

Off-target EffectGeneticsGene EditingGenome EditingGenomics +5

Crizotinib in <i>ROS1</i>-Rearranged Non–Small-Cell Lung Cancer

Alice T. Shaw, Sai‐Hong Ignatius Ou, Yung‐Jue Bang, +18

New England Journal of Medicine

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

Benjamin P. Kleinstiver, Michelle S. Prew, Shengdar Q. Tsai, +9

Nature

EngineeringSynthetic BiologyGenetic EngineeringMolecular BiologyGenome Engineering +6

A Pyrosequencing Study in Twins Shows That Gastrointestinal Microbial Profiles Vary With Inflammatory Bowel Disease Phenotypes

Benjamin P. Willing, Johan Dicksved, Jonas Halfvarson, +7

Gastroenterology

DysbiosisGut MicrobiologyGastroenterologyTwins ShowsPyrosequencing Study +6

Anchored multiplex PCR for targeted next-generation sequencing

Zongli Zheng, Matthew Liebers, Boryana Zhelyazkova, +11

Nature Medicine

Anchored Multiplex PcrNext-generation SequencingGeneticsOmicsGenomics +4

Ex vivo culture of circulating breast tumor cells for individualized testing of drug susceptibility

Min Yu, Aditya Bardia, Nicola Aceto, +21

Science

Breast OncologyPathologyCell CultureTumor BiologyOncology +19

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

Benjamin P. Kleinstiver, Michelle S. Prew, Shengdar Q. Tsai, +4

Nature Biotechnology

Pam RecognitionOff-target EffectEngineeringMedicineGenetic Engineering +8

Recurrent and functional regulatory mutations in breast cancer

Esther Rheinbay, Prasanna Parasuraman, Jonna Grimsby, +30

Nature

Breast OncologySomatic VariantBreast CancerCancer GeneticsSystems Biology +2

Incidence of gastric cancer among patients with gastric precancerous lesions: observational cohort study in a low risk Western population

Huan Song, Isabella Ekheden, Zongli Zheng, +3

BMJ