Concepedia

CRISPR off-target analysis in genetically engineered rats and mice

Keith R. Anderson, Maximilian Haeussler, Colin Watanabe, Vasantharajan Janakiraman, Jessica Lund, Zora Modrušan, Jeremy Stinson, Qixin Bei, Andrew Buechler, Charles Yu,

Nature Methods · 2018 · 233 citations · 22 references

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22

Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

Jian Ye, George Coulouris, Irena Zaretskaya et al. · BMC Bioinformatics · 2012 · 6.2K citations · Full text

, , +12

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

John G. Doench, Nicolò Fusi, Meagan E. Sullender et al. · Nature Biotechnology · 2016 · 5K citations

, , +13

DNA targeting specificity of RNA-guided Cas9 nucleases

Patrick D. Hsu, David A. Scott, Joshua A. Weinstein et al. · Nature Biotechnology · 2013 · 4.9K citations · Full text

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High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

Benjamin P. Kleinstiver, Vikram Pattanayak, Michelle S. Prew et al. · Nature · 2016 · 2.7K citations · Full text

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Rationally engineered Cas9 nucleases with improved specificity

Ian M. Slaymaker, Linyi Gao, Bernd Zetsche et al. · Science · 2015 · 2.5K citations

, , +15