High-performance liquid chromatography analysis of phytic acid on a pH-stable, macroporous polymer column

Abstract

A procedure was developed that enables up to 60 samples containing phytic acid to be analyzed in one day. Extraction time was reduced to 1-3 min per sample by using ultrasonic irradiation. Concentration of the extract was simplified by using a commercially available silica-based, anion-exchange column. High-performance liquid chromatography analysis of the concentrated sample on a macroporous polymer column was accomplished in 8 min. The column easily resolved mixtures containing inositol hexaphosphate, inositol pentaphosphate, and inositol tetraphosphate. Samples of wheat bran as small as 50 mg were analyzed by this system.