Genome biology · 2015 · 101 citations · 7 references
Life SciencesGeneticsMolecular BiologyGenetic EngineeringGenome EditingGenome EngineeringCrispr-based EngineeringCrispr-cas SystemsTarget Site SelectionCrispr-based Genome EngineeringCrispr-cas Genome EditingCrispr-cas9 Genome Editing
CRISPR-Cas systems have immense biotechnological utility. A recent study reveals the potential of the Cpf1 nuclease to complement and extend the existing CRISPR-Cas9 genome-editing tools.
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A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Martin Jínek, Krzysztof Chylinski, Ines Fonfara et al. · Science · 2012 · 17.3K citations · Full text
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
Bernd Zetsche, Jonathan S. Gootenberg, Omar O. Abudayyeh et al. · Cell · 2015 · 4.9K citations · Full text
In vivo genome editing using Staphylococcus aureus Cas9
F. Ann Ran, Le Cong, Winston X. Yan et al. · Nature · 2015 · 2.7K citations · Full text
Engineered CRISPR-Cas9 nucleases with altered PAM specificities
Benjamin P. Kleinstiver, Michelle S. Prew, Shengdar Q. Tsai et al. · Nature · 2015 · 1.7K citations · Full text
Orthogonal Cas9 proteins for RNA-guided gene regulation and editing
Kevin M. Esvelt, Prashant Mali, Jonathan L. Braff et al. · Nature Methods · 2013 · 806 citations · Full text