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Directed Evolution of a Lysosomal Enzyme with Enhanced Activity at Neutral pH by Mammalian Cell-Surface Display

Cellular EnzymologyBiochemistryProtein FoldingNatural SciencesEnzyme CatalysisDirected EvolutionMolecular BiologyEnzyme SpecificityProtein EngineeringLysosomal EnzymeStructure-function Enzyme KineticsEnzymatic ModificationMammalian Cell-surface Display