Concepedia

Directed Evolution of a Lysosomal Enzyme with Enhanced Activity at Neutral pH by Mammalian Cell-Surface Display

Kai‐Chuan Chen, Chia‐Hung Wu, Chuan-Yuan Chang, Weicheng Lu, Qingzong Tseng, Zeljko M. Prijovich, W Schechinger, Yen‐Chywan Liaw, Yu-Lin Leu, Steve R. Roffler

Chemistry & Biology · 2008 · 33 citations · 47 references

DOI

Concepts

References

47

Rapid evolution of a protein in vitro by DNA shuffling

Willem P.C. Stemmer · Nature · 1994 · 1.9K citations

, , +10

Randomization of genes by PCR mutagenesis.

R C Cadwell, Gerald F. Joyce · Genome Research · 1992 · 1.1K citations · Full text

, , +17

Tumor chemosensitivity conferred by inserted herpes thymidine kinase genes: paradigm for a prospective cancer control strategy.

Frederick L. Moolten · PubMed · 1986 · 972 citations

, , +16

Conjugation of antibodies with fluorochromes: Modifications to the standard methods

James W. Goding · Journal of Immunological Methods · 1976 · 743 citations

, , +11

Transfer of the bacterial gene for cytosine deaminase to mammalian cells confers lethal sensitivity to 5-fluorocytosine: a negative selection system.

Craig A. Mullen, Mogens Kilstrup, R. Michael Blaese · Proceedings of the National Academy of Sciences · 1992 · 505 citations

, , +14