Concepedia

Directed Evolution Screening

Directed Evolution Screening is a high-throughput screening methodology used in enzyme engineering that iteratively selects functional variants from a genetically diverse library through successive rounds of mutagenesis, expression, and functional assay.

282

Publications

14.4K

Citations

1.4K

Authors

412

Institutions

Publications per year

2017–2026

110

Parents

Publications

282

Discovery and Directed Evolution of a Glyphosate Tolerance Gene

Linda A. Castle, Daniel L. Siehl, Rebecca Gorton et al. · Science · 2004 · 327 citations

, , +12

Discovery, Characterization, Engineering, and Applications of Ene-Reductases for Industrial Biocatalysis

Helen S. Toogood, Nigel S. Scrutton · ACS Catalysis · 2018 · 310 citations · Full text

, , +17

Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization

Amir Aharoni, Leonid Gaidukov, Shai Yagur et al. · Proceedings of the National Academy of Sciences · 2003 · 288 citations · Full text

, , +17

Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening

Ji-Hu Zhang, Glenn Dawes, Willem P.C. Stemmer · Proceedings of the National Academy of Sciences · 1997 · 274 citations

, , +15

A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution

Ali Fallah-Araghi, Jean‐Christophe Baret, Michaël Ryckelynck et al. · Lab on a Chip · 2012 · 261 citations

, , +16

1–5 of 282

Authors

1.4K

  1. US
    Ulrich Schwaneberg

    RWTH Aachen University, Germany

    21 publications · 974 citations

    18

    h-index

  2. FH
    Frances H. Arnold

    California Institute of Technology, United States

    11 publications · 938 citations

    10

    h-index

  3. FH
    Florian Hollfelder

    University of Cambridge, United Kingdom

    8 publications · 229 citations

    8

    h-index

  4. SG
    Stephen G. Withers

    University of British Columbia, Canada

    7 publications · 565 citations

    7

    h-index

  5. RM
    Ronny Martínez

    RWTH Aachen University, Germany

    5 publications · 234 citations

    5

    h-index

1–5 of 1.4K

Institutions

412

  1. RWTH Aachen University

    Aachen, Germany

    18 h-index

    91 publications

    4.3K citations

  2. Chinese Academy of Sciences

    Beijing, China

    14 h-index

    78 publications

    3.1K citations

  3. University of British Columbia

    Vancouver, Canada

    11 h-index

    39 publications

    3.5K citations

  4. California Institute of Technology

    Pasadena, United States

    11 h-index

    53 publications

    4.5K citations

  5. DWI – Leibniz Institute for Interactive Materials

    Aachen, Germany

    10 h-index

    19 publications

    574 citations

  6. Max-Planck-Institut für Kohlenforschung

    Mülheim, Germany

    8 h-index

    32 publications

    2.7K citations

  7. University of Cambridge

    Cambridge, United Kingdom

    8 h-index

    27 publications

    791 citations

  8. Tianjin Institute of Industrial Biotechnology

    Tianjin, China

    7 h-index

    31 publications

    1K citations

1–8 of 412

Venues

  1. Protein Engineering Design and Selection

    PEDS · 22 publications · 1.1K citations

    18

    h-index

  2. Proceedings of the National Academy of Sciences

    PNAS · 15 publications · 1.9K citations

    14

    h-index

  3. ChemBioChem

    13 publications · 414 citations

    13

    h-index

  4. ACS Synthetic Biology

    10 publications · 363 citations

    10

    h-index

  5. Biotechnology and Bioengineering

    11 publications · 426 citations

    10

    h-index

1–5