Technical Note: A Rapid Lipid Separation Method for Determining Fatty Acid Composition of Milk

Abstract

A rapid method for milk lipid separation followed by transmethylation to produce fatty acid methyl esters from bovine milk samples is presented. Fat is separated by a nonsolvent method using centrifugation. The method was compared with the popular hexane:isopropanol solvent extraction method, and fatty acid proportions were statistically identical for both methods. In 108 replicates, variance accounted for by using the 2 methods was of a similar magnitude to variance due to repeat separations or repeat injections onto the gas chromatography column. It is concluded that the proposed method is accurate, simple, rapid, safe, economical, and especially suitable for large numbers of samples.