Journal of Bacteriology · 2010 · 50 citations · 8 references
Life SciencesBioinformaticsMicrobiologyBacteriologyGenome AssemblyGenomicsDe Novo Genome AssemblyDna SequencingGenome SequencingSequence AlignmentSequence AssemblyShort-read AssemblyBacterial Genome AssemblyGenome ReconstructionPathogen Genome AssemblyProkaryotic Sequence AnnotationWhole-genome AlignmentWhole-genome Sequence Analysis
This work reports the completion and annotation of the genome sequence of Corynebacterium pseudotuberculosis I19, isolated from an Israeli dairy cow with severe clinical mastitis. To present the whole-genome sequence, a de novo assembly approach using 33 million short (25-bp) mate-paired SOLiD reads only was applied. Furthermore, the automatic, functional, and manual annotations were attained with the use of several algorithms in a multistep process.
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