Journal of Food Science · 1980 · 18 citations · 13 references
AgricultureBiotechnologyFood ScienceBioseparationsFood ChemistryProtein EngineeringProduct PurificationProtein AnalysisProtein PurificationProtein SeparationProteins In FoodsWhey ProteinsIsoelectric PrecipitationLysine ContentProtein ExtractionProtein Separation TechniquesSeed Protein ExtractionSeed Protein SeparationWhey Protein IsolatesWhey Protein Isolation
ABSTRACT An improved process employing a combination of isoelectric precipitation and ultrafiltration to isolate glandless cottonseed protein was developed. The effectiveness of Ca(OH) 2 , NaOH, and KOH in extracting storage protein (SP) was compared at pH 10, 9.5 and 9.0. KOH and NaOH each solubilized ca 88% of the flour nitrogen at pH 10, 87% at pH 9.5, and 85% at pH 9.0. Ca(OH), proved less effective, solubilizing about one‐fifth as much as the other two hydroxides. Nonstorage protein (NSP) extract was separated by acid precipitation into curd and whey: The lysine‐rich NSP whey was then added to SP extract and the mixture ultrafiltered to obtain a higher yield of SP isolate having a lighter color and increased lysine content.
13
Colorimetric Method for Determination of Sugars and Related Substances
Michel Dubois, K. A. Gilles, J. K. Hamilton et al. · Analytical Chemistry · 1956 · 51.8K citations
Agriculture, Chemistry, Food Science +13
A Method for the Colorimetric Determination of Phosphorus
James B. Sumner · Science · 1944 · 759 citations